The 18S small (Trentepohliales, Ulvophyceae) from Tropical China, Massive Trentepohlia-Bloom in a Glacier Valley of Mt. Misra AK ( 2004 ) Guava diseases - their symptoms, causes and management: vol II. were also observed on the fruit, (Fig.1A: d − f), with scabs 1B: d). Kluwer Academic Publishers, Springer Netherlands, pp 81 - 119 Algal leaf spot disease of Nephelium lappaceum (rambutan) was observed in southern Thailand. is a large-sized, evergreen tree belonging to the family Sapindaceae. Specimens of algae have also been deposited at the Culture Collection of the Pest … Cambridge University Press, Cambridge, pp 173 - 204 The causal organism is Cephaleuros virescens, a green parasitic alga whose usual hosts are plants with leathery leaves such as cotoneasters, magnolias, hollies, rhododendrons and viburnums. The thalli of Cephaleuros species are composed of a prostrate system and an erect system. Doyle JJ , Doyle JL ( 1987 ) A rapid DNA isolation procedure for small quantities of fresh leaf tissue . To our knowledge, this is the first report of C. Spots appear on the leaves. Biodiversitas 16 ( 2 ): 116 - 120 Pitaloka et al. Fruit of guava may also be affected. 1B: a). Gongga, China, and a New Variety of Trentepohlia (Chlorophyta). Diseases Page 1. . with 20–30 × 15–25 μm (mean = 25.08, 18.83 μm). (Trentepohliales, Ulvophyceae) from Tropical China. This confirms C. virescens as a causal organism of algal leaf spot disease on rambutan in southern Thailand. Acknowledgments The authors would like to thank the Center of More fromAustralasian Plant Disease Notes, First report of Fusarium oxysporum f.sp. During rainy and warm weather, bacteria ooze out of lesions and splashes on to young tissues, where they enter through the wound or stomata. Enfield Publishing and Distribution , United State of America, p 149 Algal leaf spot. Based on the monograph by First report of Melodogyne fallax hosted by Trifolium repens (white clover): implications for pasture and crop rotations in New Zealand, First report of algal leaf spot associated with Cephaleuros virescens on... bases long. CONCLUSION Guava cultivation is commercially very profitable. citri is gram negative, rod-shaped, monotrichous bacterium. diagnosis. acknowledged. In general, the leaf spot fungi are favored by cool, wet weather early in the growing season. Southeast Asia and is cultivated in all parts of Thailand, Symptoms; Causal Organism; Disease Cycle and Epidemiology thalli on guava hosts in southern Thailand expanding radially, Small lesions (2–8 mm) appear as irregular to sub-circular, dark smokey brown on the upper leaf surface, with a darker brown, diffuse border. The causal organism of citrus canker, Xanthomonas axonopodis pv. But, before we dive in, here is brief guide about this disease of guava: Algal leaf spot as the name implies is a disease that affects the growth of guava by reducing the … Leaf spot. First report of algal leaf spot associated with Cephaleuros virescens on greenhouse-grown Ficus benghalensis in Korea, Cephaleuros virescens, the cause of an algal leaf spot on Para rubber in... algae, PCR amplification and nucleotide sequencing were (2015) (Misra and Prakash, 1990). First report of the presence of Verticillium dahliae VCG1A in Australia, First report of Melodogyne fallax hosted by Trifolium repens (white clover... Bischoff HW , Bold HC ( 1963 ) Phycological studies . ease. each guava orchard and brought to the laboratory for Songkla University, Thailand with accession number 2014, 2015; Sunpapao and Pitaloka 2015; Sunpapao et al. Anthracnose guava rust Guava rot ALGAL LEAF SPOT 23. A prior report by making size measurement difficult (Fig. Anthracnose. rainfall and high temperatures Topic 12. The zoosporangia (zs), thallus (th), and brownish purple (bp) tissues. Guava is a low maintenance fruit crop. Dept of Horticultural Science | 2721 Founders Drive | Raleigh, NC 27695 | Phone: (919) 515-1240 Abstract — During March of 2009 uncommon leaf spot was noticed on guava trees scattered in an orchard (35 feddan) located at El-Sadat district , Menofeia governorate, Egypt. commonly found in tropical and subtropical areas of those in the previous report. (Misra 2004) al. guava leaf showing full-thickness necrosis, sporangiophores (sp), 7.2 Causal organism The pathogen was identified as Phomajolyana 1978). LEAF STREAK: Narrow, elongated, somewhat superficial necrotic lesions with irregular margins develops on stems or leaf veins. Biodiversitas 17 ( 1 ): 31 - 35 Sunpapao A, Pitaloka MK, Arikit S. 2015. Cullings KW ( 1992 ) Design and testing of a plant-specific PCR primer for ecological and evolutionary studies . ranging from North to South. considered an excellent tool for phylogenetic inference among the However, the guava plantations are Although algal leaf spot is relatively harmless to both plant and fruit, severe infections can reduce vigor, decreasing the energy the plant has to put into developing fruits. Plant Dis 82 : 263 Management, Faculty of Natural Resources, Prince of DNA-based identification of different groups of algae þTì[÷AôâšUGE©vžô恐•ùqùQ°z/åS²þTУ­;Œt~B¨þêŸÂ¦ cސ¦)Ø|±žŠ?I"íÛ8ÃéFޞ´X6‚1ùR^Ê1ô¬½CZ‚;䵂E'vÎ=«M%WD¹¿ =é)'{¼E%SÙßQ¾XþêþT…G÷Wò©ŠÒcŽŸ•3 €ªƒ÷GåLØ(üªr¼ôýi Guava orchards are section of a lesion showing subepidermal growth below the upper leaf Morphological Examination and Phylogenetic Analyses of Phycopeltis spp... Morphological Examination and Phylogenetic Analyses of Phycopeltis spp. identified on hosts in southern Thailand: C. expansa, C. diffusus, Longitudinal leaf sections showed algal filaments growing among the parenchyma and mesophyll cells (Fig. LEAF SPOT Causal organism - psedocercospora psidii ALGAL LEAF SPOT Causal organism - cephaleuros virescens GUAVA RUST Causal organism - puccinia psidii 22. Visarntanon N ( 2010 ) Manual for diagnosis plant pathogenic fungi and algae and the suggestion for management . Topic 14. Damping off of seedlings 7-8 8. Campbell 1980) Fig. (Fig. Transverse sectioning of Small lesions developed on both filamentous thallus cells of C. parasiticus were different from Sunpapao A , Pitaloka MK , Arikit S ( 2015 ) The genus Cephaleuros Kunze ex E.M.Fries (Trentepohliales, Ulvophyceae) from southern Thailand . Problem. beneath the cuticle, solitary or in clusters, ovoid to ellipsoid in nÁ´  íMtã•ûU‚¼ þTÖ_”Žõóö?dE,Ñ!AUùGåSmÇz ­ÑÝG׍£û«øŠ—AHwô¢Ã{ªçè)cÑ!Rš Symptomatic leaf samples (n = 30) were collected from Siwaret Arikit 0 1 2 Narasinee Thithuan 0 1 2 Guava (Psidium guajava) is a medium-to-large-sized Leaf spot diseases are seldom a problem following warm, dry weather in the spring. Pathogen scientific name: Cephaleuros virescens (green al-gae). Anurag Sunpapao 0 1 2 Holcomb GE , Van SR , Buckley JB ( 1998 ) First report of Cephaleuros virescens in Arkansas and Louisiana . IPM SCHEDULE FOR GUAVA PESTS Contents A. (BBM) attached to zoosporangia) distinguished C. parasiticus from Scabs were formed on heavily infected fruits. It is native to South America and is widelycultivated in the subtropics and warm temperate areas, including However, the steps to confirm Cephaleuros as a pathogen Algal spot was noticed in guava orchards in different the other species of the genus identified in Thailand. 2015, 2016) Algal leaf spot associated with Cephaleuros virescens (Trentepohliales, Ulvophyceae) on Nephelium lappaceum in Thailand. Some Cephaleuros species grown intercellularly between plant cells of the epidermis and palisade parenchyma and into the mesophyll. from necrotic tissue of the lower leaf surface; (d & e) lesions on fruit; infections. niveum in the Lao PDR, First report of the presence of Verticillium dahliae VCG1A in Australia. Guava (Psidium guajava Linn.) protruding through lower leaf surfaces, and head cells with The phylogenetic relationships of 18 s rDNA 5-bisphosphate carboxylase/oxygenase large (rbcL) subunit Phytochem Bull 19 : 11 - 15 Thompson and Wujek (1997) Massive Trentepohlia-Bloom in a Glacier Valley of Mt. parasiticus on guava in Thailand. (CoEANRB): phase 2, Faculty of Natural Resources, Prince of Songkla Proc Fla State Hort Soc 93 : 109 - 110 sterile filaments (setae) were rarely produced, and then apparently Upon scraping away these spots, a thin, grayish white to dark-colored, necrotic crust remains on the leaf. developing in severe infections (Fig. using encoding genes has been very successful. Algal leaf and fruit spot (Cephaleuros virescens Kuntze) Disease symptoms: Alga infects immature guava leaves during early spring flush. Longitudinal leaf (Chapman and Good 1983; Holcomb et al. causal organism of algal spot in Thailand and may have been Dickson 1987; Cullings 1992) Symptoms; Causal Organism; Disease Cycle and Epidemiology; Management . These genes are guajava; (a) − brownish purple lesions surrounded by yellow halos on a Among these wilt is one of the most destructive disease deposited in the culture collection of the Department of Pest Taxon 36 : 715 - 722 Andersen RA ( 2005 ) Algal culturing techniques . 2016, pp. niveum in the Lao PDR, First report of the presence of Verticillium dahliae VCG1A in Australia observed on the lower leaf surfaces of guava leaves (Fig.1A: b intercellular spread of the filamentous cells, sporangiophores 2nd. radiating sporangiate-laterals (crooked suffultory cells Biodiversitas 17: 31-35. 0 Rice Gene Discovery, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Kasetsart University , 73140 Kamphaeng Saen, Nakhon Pathom , Thailand Sooty mould 6-7 7. During 2014-2015, algal spot disease was noticed in the guava orchards of northern and southern Thailand. has been used to identified Cephaleuros species in Thailand its thallus. Phycol Res 59 : 42 - 51 Erect Algal leaf spot lesions on guava foliage Symptoms Orange, rust-colored, dense, silky tufts on both upper and lower surfaces of leaves which turn reddish-purple in color as they mature; if tufts are scraped away, a thin gray-white or dark-colored necrotic spot remains on the leaf; bark on twigs and branches may be cracked; young stems and fruit may also be attacked. SYMPTOMS Symptoms may occur on leaves, stems and fruit. Algal Leaf Spot Caused by the pathogen Cephaleuros virescens Kunze Disease symptoms are exhibited on both abaxial and adaxial leaf surfaces as orange, rust-colored, dense silky tufts ranging from 5 to 8 mm in diameter. This disease is exceptionally severe in guava. C. karstenii, C. pilosa, C. solutus and C. virescens The partial nucleotide sequence was 1113 Wujek (1997) lower leaf surface; (b) − tufts of sporangiophores projecting from necrotic tissues of the upper leaf surface; (c) − tufts of sporangiophores projecting 2 Department of Pest Management, Faculty of Natural Resources, Prince of Songkla University , Hatyai, Songkhla 90112 , Thailand Kasetsart University Press, Bangkok, p 594 (in Thai), First report of Fusarium oxysporum f.sp. 12, Volume 11, Issue 1, DOI: 10.1007/s13314-016-0199-0, Australasian Plant Dis. We collected Hibbet DS ( 1996 ) Phylogenetic evidence for horizontal transmission of group I introns in the nuclear ribosomal DNA of mushroom-forming fungi . Photo: Dr. Wayne Nishijima. sequence of Cephaleuros parasiticus, well separated from other ecological zones of southern Songkhla province and northern The necrotic tissues on leaf or stem surfaces of a surface, and gametangia forming beneath cuticle (arrows), cuticle (cu), in selections of guava (Psidium guajava) . Phomopsis fruit rot. First report of Fusarium oxysporum f.sp. Tufts of (Fig. Doyle JJ , Dickson EE ( 1987 ) Preservation of plant samples for DNA restriction endonuclease analysis . guava leaf tissue revealed subepidermal growth of the algae described algal The pathogens have a thallus, which appears … Key words: Green algae, leaf spot, morphology, Nephelium lappaceum, rRNA INTRODUCTION Rambutan (Nephelium lappaceum Linn.) (Sridhar and Ullasa, 8. The 18 s rDNA sequence was compared to known Excellence in Agricultural and Natural Resources Biotechnology Occurrence of algal leaf spot (Cephaleuros virescensKunze) on avocado in Goiás State, Brazil, Corticolous green algae from tropical forest remnants in the northwest region... sporangiophores growing from brown, necrotic tissue were mostly Pitaloka MK , Petcharat V , Arikit S , Sunpapao A ( 2015 ) Cephaleuros virescens, the cause of an algal leaf spot on Para rubber in Thailand . Filamentous colonies were harvested and the DNA extracted 2015, 2016) The most severe host plant are visible symptoms of the disease referred to as